| 128 | Karina Elvestrom BYE |
| 128 | Savannah Rosenhan (GAC) def. Izzy Brinkman (UWLC) 6-7, 6-3, 10-7 |
| 128 | Ella De Young BYE |
| 128 | Ashley Adkisson (UWW) def. Katie Thompson (GAC) 6-4, 6-1 |
| 128 | Rika Cross (GAC) def. Alexandra Berns (STO) 6-1, 6-4 |
| 128 | Madison Packer (GAC) def. Kate Trifilio (CARROLL) 6-3, 6-1 |
| 128 | Maya Brakhage BYE |
| 128 | Lanie Davis BYE |
| 128 | Noor Omar BYE |
| 128 | Pavla Yakimova BYE |
| 128 | Kaya De Bruijn BYE |
| 128 | Allison Szalay BYE |
| 128 | Molly Austin BYE |
| 128 | Lily Jorgenson (UWLC) def. Bethany Smith (GAC) 6-2, 6-1 |
| 64 | Karina Elvestrom (GAC) def. Elif Oezen (UWS) 6-0, 6-0 |
| 64 | Abby Ostermann (LUT) def. Savannah Rosenhan (GAC) 6-3, 2-6, 10-8 |
| 64 | Ella De Young (GAC) def. Tannishtha Mukherjee (GRIN) 7-5, 6-2 |
| 64 | Pari Chaven (GRIN) def. Rika Cross (GAC) 6-1, 6-0 |
| 64 | Julia Moreno (LUT) def. Maya Brakhage (GAC) 7-6, 2-6, 10-5 |
| 64 | Xingchen Liu (CARTH) def. Madison Packer (GAC) 6-2, 6-2 |
| 64 | Lanie Davis (GAC) def. Aubrey Wittwer (UEWC) 6-0, 5-7, 10-6 |
| 64 | Noor Omar (GAC) def. Faith Simon (SCU) 6-1, 6-3 |
| 64 | Rafaela Tavares (LUT) def. Pavla Yakimova (GAC) 4-6, 7-5, 10-5 |
| 64 | Kaya De Bruijn (GAC) def. Greta Peterson (CART) 6-0, 6-1 |
| 64 | Eliana Martin (GRIN) def. Allison Szalay (GAC) 6-3, 1-6, 10-8 |
| 64 | Molly Austin (GAC) def. Reese Burton (UWEC) 6-2, 6-0 |
| 32 | Karina Elvestrom (GAC) def. Addison Leasure (COE) 6-2, 6-2 |
| 32 | Michaella Sullivan (BU) def. Ella De Young (GAC) 7-5, 6-4 |
| 32 | Kiera Healy (GRIN) def. Lanie Davis (GAC) 3-6, 7-6, 11-9 |
| 32 | Noor Omar (GAC) def. Anika Sheth (GRIN) 7-5, 6-0 |
| 32 | Kaya De Bruijn (GAC) def. Luiza Kohn (GRIN) 6-2, 6-0 |
| 32 | Molly Austin (GAC) def. Logan Tom (GRIN) 6-2, 6-1 |
| 16 | Karina Elvestrom (GAC) def. Abby Ostermann (LUT) 6-1, 6-1 |
| 16 | Noor Omar (GAC) def. Gracie Ha (UWW) 6-4, 6-1 |
| 16 | Kaya De Bruijn (GAC) def. Chloe Regal (UWSP) 6-3, 6-2 |
| 16 | Molly Austin (GAC) def. Shelby Roth (UWLC) 5-7, 6-3, 10-8 |
| 8 | Karina Elvestrom (GAC) def. Tasha Bailey (UWLC) 6-0, 6-0 |
| 8 | Kaya De Bruijn (GAC) def. Noor Omar (GAC) 6-1, 4-6, 6-1 |
| 8 | Lauryn Douglas (BU) def. Molly Austin (GAC) 6-2, 6-0 |
| 4 | Karina Elvestrom (GAC) def. Xingchen Liu (CARTH) 6-2, 6-0 |
| 4 | Kaya De Bruijn (GAC) def. Lauryn Douglas (BU) 4-6, 6-1, 6-4 |
| 2 | Karina Elvestrom (GAC) def. Kaya De Bruijn (GAC) 6-4, 2-6, 6-2 |
| D1-1 | Alliandra Hagness (COE) def. Sidney Wright (GAC) 8-3 |
| D2-1 | Annie Klemmensen (GAC) def. Lisey Langhus (BU) 8-3 |
| D2-2 | Doyeon Kim (GRIN) def. Annie Klemmensen (GAC) 8-6 |
| D3-1 | Melat Tabara (GAC) def. Delaney Hanson (UWEC) 8-5 |
| D3-2 | Melat Tabara (GAC) def. Logan Kane (LAW) 8-2 |
| D3-3 | Melat Tabara (GAC) def. Emily Dreger (HU) 8-4 |
| D3-4 | Melat Tabara (GAC) def. Claudia Curtis (UWS) 8-2 |
| D4-1 | Anna Key (GAC) def. Tess McGlenn (LAW) 8-2 |
| D4-2 | Anna Key (GAC) def. Olivia Walter (CC) 8-3 |
| D4-3 | Anna Key (GAC) def. Ava Pellegrino (UWEC) 8-2 |
| D4-4 | Maria Bello (COE) def. Anna Key (GAC) 8-2 |
| D5-1 | Taylor Ruhl (GAC) def. Marcela Cortez (CSS) 8-2 |
| D5-2 | Morgan Mila (UWSP) def. Taylor Ruhl (GAC) 7-7 |
| D5-3 | Chloe Reyes (HU) def. Taylor Ruhl (GAC) 8-2 |
| D7-1 | Evelyn Bryson (GAC) def. Stella Platt (UWEC) 8-3 |
| D7-2 | Evelyn Bryson (GAC) def. Ellie Huber (CSB) 8-7 |
| D7-3 | Emma Kappel (UWLC) def. Evelyn Bryson (GAC) 8-4 |
| D8-1 | Ashley Stroebel BYE |
| D8-2 | Ashley Stroebel (GAC) def. Erin Horzewski (CARROLL) 8-4 |
| D8-3 | Alice Tysoe (COE) def. Ashley Stroebel (GAC) 8-0 |
| D9-1 | Jemima Vitou BYE |
| D9-2 | Jemima Vitou (GAC) def. Dana Keesler (STO) 8-6 |
| D9-3 | Jemima Vitou (GAC) def. Kaitlyn Narkis (EDG) 8-1 |
| D9-4 | Jemima Vitou (GAC) def. Audrey Smal (CORN) 8-4 |
