| 128 | Alyssa Trapp (GC) def. Bethany Smith (GACW) 2-6, 7-5, 11-9 |
| 128 | Kaya De Bruijn (GACW) def. Liv Herzog (UWEC) 6-1, 6-1 |
| 128 | Brooke Haddorff (GACW) def. JennaMarie Hernandez (CEN) 6-1, 6-1 |
| 128 | Riley O'Donnell (LUT) def. Ella De Young (GACW) 6-2, 4-6, 10-3 |
| 64 | Kaya De Bruijn (GACW) def. Cecile Fuchs (EDGE) 6-4, 6-4 |
| 64 | Allison Szalay (GACW) def. Sarah Ferguson (UWW) 6-3, 1-5, retired |
| 64 | Pavla Yakimova (GACW) def. Ashley Adkisson (UWW) 6-3, 4-6, 19-17 |
| 64 | Alli Laux (GACW) def. Kate Townsend (UWL) 6-1, 6-3 |
| 64 | Brooke Haddorff (GACW) def. Sydney Ellison (MAC) 5-7, 7-5, 6-6, by default |
| 64 | Molly Austin (GACW) def. Elizabeth Trevathan (MAC) 6-1, 6-1 |
| 32 | Julia Maier (CSS) def. Kaya De Bruijn (GACW) 6-2, 7-6 (5-0) |
| 32 | Allison Szalay (GACW) def. Anna Lee (UWEC) 6-3, 7-6 (5-0) |
| 32 | Pavla Yakimova (GACW) def. Paige Sargent (GC) 7-5, 7-6 (7-0) |
| 32 | Alli Laux (GACW) def. Isa Voinescu (GC) 6-4, 6-2 |
| 32 | Cassandra Lee (UWW) def. Brooke Haddorff (GACW) 6-2, 6-1 |
| 32 | Molly Austin (GACW) def. Shelby Roth (UWL) 6-0, 7-6 (3-0) |
| 16 | Ricki Budewitz (CUW) def. Allison Szalay (GACW) 0-6, 7-6 (4-0), 10-7 |
| 16 | Michaella Sullivan (BU) def. Pavla Yakimova (GACW) 6-3, 6-1 |
| 16 | Ionna Giannakou (GC) def. Alli Laux (GACW) 6-7 (0-7), 6-3, 10-8 |
| 16 | Molly Austin (GACW) def. Sofia Sackett (LUT) 6-4, 6-0 |
| QF | Lauryn Douglas (BU) def. Molly Austin (GACW) 6-0, 7-5 |
